E-mail: eprapplications@bruker-biospin.com FAX: 978-670-8851 Tel. 978-663-7406 mailing address EPR Division Bruker BioSpin Corporation 19 Fortune Drive Manning Park Billerica, MA 01821 USA Thank you for your help. Quantitative EPR experiments; Bruker e-scan EPR spectrometer. The Bruker e-scan is a compact benchtop EPR spectrometer. A multi-purpose version is available, as well as dedicated analysers for specific real-world applications. The e-scan is a reliable EPR system, optimised for the best price-performance ratio.
2D HYperfine Sub-level CORrElation
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2D HYSCORE is essentially a two dimensional ESEEM experiment in which correlation is transferred from one electron spin manifold to another.
In a HYSCORE experiment, the time between the second π/2 and π pulse is varied in one dimension and the time between the π and third π/2 pulse is varied in a second dimension. A two dimensional FT gives the sample data below.
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2D-HYSCORE Spectrum of a Coal Standard Sample
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As in an ESEEM experiment, the peaks observed are essentially an NMR spectrum of nuclei that are coupled to the electron. HYSCORE allows one to take a complicated ESEEM spectrum and extend the data into a second dimension Peaks appearing in the upper right and lower left quadrants typically arise from nuclei in which the hyperfine coupling is less than the larmor frequency. They appear at the larmor frequency, separated by the hyperfine coupling. Peaks from nuclei in which the hyperfine interaction is greater than the larmor frequency appear in the upper left and lower right quadrants. The HYSCORE spectrum can get very complicated for nuclei with a spin of I > 1/2 with many peaks arising from the complication of additional nuclear zeeman and quadrupole levels. Even with the complexity of the spectra, HYSCORE on systems with multiple nuclei can make ESEEM spectra that would be difficult or impossible to interpret much more manageable.